Education

• BS Yamagata University, Yamagata, Japan 1973
• Ph.D. Tohoku University School of Medicine, Bacteriology, Sendai, Japan 1982

Research Interests

• Mechanisms regulating ontogenic and functional heterogeneity of macrophage populations.
• Macrophage-mediated regulation of innate and acquired immune responses.
• Intracellular trafficking of particles and activation of cellular signaling in phagocytosis.
• Development of a new Th1 adjuvant against infections and allergic asthma.
• Mechanisms of chronic inflammation in atheroscelerosis.

Professional Background

• East Carolina University Brody School of Medicine, Greenville, NC. Research Associate Professor, 1996 - 2003.
• George Mason University, Medical Sciences Research Inst, Herndon, VA. Associate Scientist/Associate Professor, 1994-1996.
• East Carolina University School of Medicine, Dept. Pathology, Greenville, NC. Research Assistant Professor, 1989 - 1994.
• Hayashibara Biochemical Laboratories, Inc., Fujisaki Cell Center, Assistant to Director, Japan, 1987-1989

Representative Peer Review Publications

• Shinohara T, T Pantuso, S Shinohara, M Kogiso, QN Myrvik, RA Henriksen, Y. Shibata. Persistent inactivation of macrophage cyclooxygenase 2 and PGE2 release in mycobacterial pulmonary inflammation. 2008 (in revision).
• Nishiyama A, T Shinohara, T Pantuso, S Tsuji, M Yamashita, Shinohara S, QN Myrvik, RA Henriksen, Y. Shibata. Depletion of cellular cholesterol enhances macrophage MAPK activation by chitin microparticles but not by heat-killed Mycobacterium bovis BCG. Am J Physiol (Cell Physiol) 295:341-349, 2008.
• Yamashita M, T Shinohara, S Tsuji, QN Myrvik, A Nishiyama, RA Henriksen, Y. Shibata. Catalytically inactive cyclooxygenase 2 (COX-2) and absence of PGE2 biosynthesis in murine peritoneal macrophages following in vivo phagocytosis of heat-killed Mycobacterium bovis BCG. J. Immunol.179:7072-7078, 2007.
• Tsuji S, M Yamashita, A Nishiyama, T Shinohara, Z. Li, QN Myrvik, DR Hoffman, RA Henriksen, Y. Shibata. Differential structure and activity between human and mouse intelectin-1: human intelectin-1 is a disulfide-linked trimer, whereas mouse homologue is a monomer. Glycobiology 17:1045-1051, 2007.
• Yamashita M, A Nishiyama, QN Myrvik, RA Henriksen, S Tsuji, Y. Shibata. Differential subcellular localization of COX-2 by macrophages phagocytosing Mycobacterium bovis BCG in vivo. Am J Physiol (Cell Physiology) 293:C184-C190, 2007.
• Shibata, Y., H Ohata, M Yamashita, S Tsuji, JF Bradfield, RA Hendrickson, A Nishiyama, QN Myrvik. Immunologic response enhances atherosclerosis -- Th1-to-Th2 shift and calcified atherosclerosis in BCG-treated apolipoprotein E-/- mice. Translational Research 149:62-69, 2007.
• Nishiyama, A, S Tsuji, M Yamashita, RA Henriksen, QN Myrvik, Y. Shibata. Phagocytosis of N-acetyl-D-glucosamine particles, a Th1 adjuvant, results in MAPK activation and TNF-α, but not IL-10, production. Cell Immunol 239:103 – 112, 2006.
• Shibata, Y., J Gabbard, M Yamashita, S Tsuji, M Smith, A Nishiyama, RA Henriksen, QN Myrvik. Heat-killed BCG induces biphasic cyclooxygenase-2+ splenic macrophage formation --- role of IL-10 and bone marrow precursors. J Leukocyte Biol 80:590-598, 2006.
• Shibata, Y., Henriksen RA, Honda I, Nakamura RM, Myrvik QN. Splenic PGE2-releasing macrophages regulate Th1 and Th2 immune responses in mice treated with heat-killed BCG. J Leukocyte Biol 78: 1281-1290, 2005.