Instruments

There are currently two flow cytometers in the core facility. The BD FACSCalibur analyzer and BD FACSAria high-speed digital bench-top cell sorter.

The BD FACSCalibur is a six parameter instrument equipped with two lasers, a 488-nm argon-ion laser and a 635-nm red diode laser, capable of detecting 4 fluorescent signals.

The BD FACSAria is a sixteen parameter instrument equipped with four fiber-optic-linked solid-state lasers, a 488-nm blue Sapphire laser, a 633-nm red HeNe laser, a 405-nm violet laser, and a 335-nm UV laser, capable of detecting 14 fluorescent signals. The FACSAria can perform two- and four- way bulk sorting for a variety of tube sizes as well as automatic cell deposition (ACDU) for sorting into multiwell plates or onto microscope slides. The FACSAria is also equipped with aerosol management (AM) and Temperature Control (TC) options.