Yoshimi Shibata, Ph.D., is a professor of biomedical science. Dr. Shibata studies a novel mechanism to understand a long-held view that activation of local macrophages by immunoactivators in vivo results in less production of PGE2 compared with untreated macrophages. PGE2 is a multifunctional inflammatory mediator, regulating local inflammation, a Th1-to-Th2 shift, airway smooth muscle relaxation, and tumor progression. His research demonstrated that activities of cyclooxygenase (COX-2), a rate-limiting enzyme for PGE2 synthesis, are dependent on in vivo but not in vitro activation of tissue macrophages. A series of COX-2/PGE2 studies support that macrophages are heterogeneous populations, demonstrating the origin of selected monocyte-independent tissue macrophages.
Dr. Shibata also studies a mechanism of phagocytosis –dependent and -independent macrophage activation using mouse models of human diseases including asthma, colitis, and mycobacterial vaccination. His studies have been cited by other groups who investigate the effects of chitin on infections caused by chitin-containing fungi and parasites, and on allergic diseases caused by chitin-containing allergens, i.e. dust mites, sea foods, and fungi.
- 1973: B.S., General Biology, Yamagata University, Yamagata, Japan
- 1982: Ph.D., Department of Bacteriology, Tohoku University School of Medicine, Sendai, Japan
- Research Area: Bacteriology, Immunology, Tumor Biology; Supervisor: Dr. Nakao Ishida
- 1983-1987: Post- Doctorate, Department of Pathology, East Carolina University School of Medicine, Greenville, North Carolina
- Research Area: Experimental Hemato-pathology, Bacteriology, Immunology; Supervisor: Dr. Alvin Volkman
- 2007-Present: Tenured Professor, Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, Florida
- 2005-2010: Affiliated Associate Professor of Immunology, Department of Microbiology, Miller School of Medicine, University of Miami, Miami, Florida
- 2003-2007: Tenured Associate Professor, Department of Biomedical Sciences, Florida Atlantic University
- 1999-2003: Research Associate and Professor, Department of Physiology, East Carolina University School of Medicine, Greenville, North Carolina
- 1995-1999: Research Associate and Professor, Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina
- 1994-1995: Associate Professor, Department of Biology, George Mason University, Fairfax, VA (Affiliate)
- 1994-1995: Associate Scientist, Medical Sciences Research Institute, Herndon, Virginia
- 1989-1994: Research Assistant and Professor, Department of Pathology, East Carolina University School of Medicine, Greenville, North Carolina
- 1987-1989: Scientist & Assistant to Director, Fujisaki Cell Center, Hayashibara Biochemical Laboratories Inc., Fujisaki, Okayama, Japan
- Davis S, Cirone AM, Menzie J, Russell F, Dorey CK, Shibata Y, Wei J, Nan C. Phagocytosis-mediated M1 activation by chitin but not by chitosan. Am J Physiol Cell Physiol. 2018 May 2. doi: 10.1152/ajpcell.00268.2017.
- Liberti A, Zucchetti I, Melillo D, Skapura D, Shibata Y, De Santis R, Pinto MR, Litman GW, Dishaw LJ. Chitin protects gut epithelial barrier in a protochordate model of DSS-induced colitis. Biol Open 2017. DOI: 10.1242/bio.029355
- Nagatani K, S Wang, V Llado, CW Lau, Z Li, A Mizoguchi, CR Nagler, Y Shibata, H-C Reinecker, JR Mora, E Mizoguchi. Chitin-microparticles for the control of intestinal inflammation. Inflammatory Bowel Diseases, 18:1698-1710, 2012.
- Tsuji S, Yamashita, DR Hoffman, T Shinohara, T Ohtsu, Y Shibata. Capture of heat-killed Mycobacterium bovis, bacillus Calmette-Guérin, by intelectin-1 deposited on cell surfaces. Glycobiology19:518-526, 2009.
- Shinohara T, M Yamashita, T Pantuso, M Kogiso, S Shinohara, QN Myrvik, RA Henriksen, Y Shibata. Persistent inactivation of macrophage cyclooxygenase-2 in mycobacterial pulmonary inflammation. Am J Resp Cell Mole Biol 41:146-154, 2009.
- Yamashita M, T Shinohara, S Tsuji, QN Myrvik, A Nishiyama, RA Henriksen, Y Shibata. Catatlycially inactive cyclooxygenase 2 (COX-2) and absence of PGE2 biosynthesis in murine peritoneal macrophages following in vivo phagocytosis of heat-killed Mycobacterium bovis BCG. J Immunol.179:7072-7078, 2007.
Shibata Y, I Honda, JP Justice, MR Van Scott, RM Nakamura, QN Myrvik. A Th-1 adjuvant --- N-acetyl-D-glucosamine polymer up-regulates Th-1 immunity but down-regulates Th-2 immunity against a mycobacterial protein (MPB-59) in interleukin-10-knockout and wild-type mice. Infect Immun 69:6123-6130, 2001.
Shibata Y, LA Foster, JF Bradfield, and QN Myrvik: Oral administration of poly N-acetyl-D-glucosamine downregulates serum IgE levels and lung eosinophilia in the ragweed-sensitized allergic mouse. J. Immunol. 164:1314-1321, 2000.
Shibata Y, LA Foster, WJ Metzger, Q N Myrvik: Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice. Infect. Immunity 65:1734-1741, 1997.
Shibata Y, A. P. Bautista, S. N. Pennington, J.L. Humes, and A. Volkman: Eicosanoid production by peritoneal and splenic macrophages in mice depleted of bone marrow by 89Sr. Am. J. Pathol., 127, 75-82, 1987.
Shibata Y, and A. Volkman: The effect of hemopoietic microenvironment on suppressor macrophages in the spleen in the congenitally anemic mice of the genotype Sl/Sld. J. Immunol., 135, 3905-3910, 1985.
Shibata Y, and A. Volkman: The effect of bone marrow depletion on prostaglandin E producing suppressor macrophages in mouse spleen. J. Immunol., 135, 3897-3804, 1985.
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Invited Talks and Honors
1998-2003: Board member of Leonard Wood Memorial American Leprosy Foundation (Rockville, MD)
2003: Invited speaker at Tokyo Medical and Dental University, Tokyo, Japan, 10/3/03.
2003: US Delegate, US-Russia Joint Symposium on the Role of Infections and Inflammatory Responses in the Heart and Lung (sponsored by NIHL), A US Delegate among 8. Orlando, FL, 11/6-11/8/03.
2004: Invited speaker, the CDMRP’s Military Health Research Forum (MHRF, sponsored by DOD), San Juan, Puerto Rico, 4/25-4/28/04.
2005: Invited speaker, NIHL Role of Infectious Agents Meeting (sponsored by NIHL), Bethesda, MD, 7/10-7/11/05
2005: Invited speaker, International Symposium on Low-Dose Radiation Exposure and Bio-defense system (sponsored by Institute for Environmental Sciences), 9/28 – 9/30/05, Rokkasho, Aomori, Japan.
2006: Invited speaker, the CDMRP’s Military Health Research Forum (MHRF, sponsored by DOD), San Juan, Puerto Rico, 5/1 – 5/4/06.
2006: Invited speaker, Florida Professionals in Infection Control (FPIC), Orlando, FL, 9/13/06.
2003-2007: Peer Review Services of research grants and manuscripts including American Heart Association and NSF.
2007: The Researcher of the Year Award – Sponsored Projects at the Associate Professor level
2008: Invited speaker, Tokushima University School of Medicine, Japan.
2008: Invited speaker, Florida International University, Miami, Florida.
Reticuloendothelial Society (Society for Leukocyte Biology)
Japanese Immunology Association (Kyoto, Japan)
Japanese Cancer Association (Tokyo, Japan)
North Carolina Thoracic Society (Raleigh, NC)
Board Member, Leonard Wood Memorial American Leprosy Foundation (Rockville, MD)
American Association of Immunologists (Bethesda, MD)
American Physiological Society (Bethesda, MD)
American Society for Microbiology (Washington, DC)
NIH R15 AT008252-01 (Yoshimi Shibata, PI) 6/1/14-5/30/18 Dietary anti-inflammatory chitin in colitis